Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (lane 1), MCF-7 (lane 2), A431 (lane 3), HT-29 (Lane 4) and HeLa (lane 5). The blots were probed with Anti-14-3-3 Sigma Mouse Monoclonal Antibody (Product # MA5-11663, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 28 kDa band corresponding to 14-3-3 Sigma was observed across cell line tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with PierceTM Power Blotter System (Product # 22834) The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).